Effect of proanf31-67 on sodium excretion in conscious monkeys.
Benjamin, B. A., and T. V. Peterson.
Dept. of Cell Biology., Duke Univ. Med. Center, Durham, NC 27710
and Dept. of Med. Physiology, Texas A&M Univ., College of
Medicine, College Station, TX 77843.
APStracts 2:0236R, 1995.
The purpose of this study was to determine the effect of proANF31-67
on renal function in conscious, chronically instrumented monkeys
(Macaca fascicularis). The experimental protocol consisted of a 30
minute control period followed by 60 minutes of peptide infusion, and
a 30 minute recovery period. ANF99-126, proANF31-67 or a combination
of ANF99-126 plus proANF31-67 were infused at 15 pmol/kg/min. ANF99
-126 caused sodium excretion to increase from 12.5+/-3 to a peak of
37.9+/-10.4 [mu]Eq/min while fractional sodium excretion (FENa)
increased from 0.69+/-0.2 to 2.3+/-0.43%. ProANF31-67 increased
sodium excretion from 12.7+/-5 to a peak of 23.3+/-9.0 [mu]Eq/min and
FENa from .56+/- .07 to a peak of 1.15+/-0.4%. When ANF99-126 and
proANF31-67 were infused in combination, sodium excretion increased
from 9.6+/-3 to a peak of 51.9+/-12.5 [mu]Eq/min while FENa increased
from 0.97+/-.4 to 3.9+/-1.1%. Mean arterial pressure decreased
approximately 10 mmHg in all three groups. These findings indicate
that intravenously administered proANF31-67 causes an increase in
sodium excretion and that when proANF31-67 and ANF99-126 are given in
combination, they act in an additive fashion to increase renal sodium
excretion.
Received 3 January 1995; accepted in final form 27 June 1995.
APS Manuscript Number R6-5.
Article publication pending Am. J. Physiol. (Regulatory Integrative
Comp. Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 15 September 1995.