Control of acid secretion in cultured gas gland cells of the
european eel anguilla anguilla.
Pelster, Bernd, and Lutz Pott.
Institut f[umlaut]ur Physiologie, Ruhr-Universit[umlaut]at Bochum,
FRG
APStracts 2:0261R, 1995.
Single cells and cell clusters isolated from the swimbladder
epithelium of the European eel Anguilla anguilla attached to collagen
S coated petri dishes and proliferated in a modified DMEM medium,
supplemented with 0.5% foetal calf serum (FCS). At a temperature of
20-22oC the growing colonies reached confluence typically within 6-8
days. Activities of glycolytic and pentose phosphate shunt enzymes
remained stable or increased only slightly during the first ten days
of primary culture. Incubated in a defined medium providing glucose
as a fuel gas gland cells in primary culture produced and released
lactic acid. The rate of acid secretion of cultured gas gland cells
measured with a cytosensor microphysiometer was not influenced by
cholinergic stimulation. Similarly, the Ca2+-ionophore A23187 had no
effect. Adrenergic stimulation with epinephrine or the [beta]-agonist
isoproterenol also did not increase the rate of acid secretion,
indicating that in gas gland cells the metabolic activity cannot be
stimulated via [beta]-adrenergic stimulation followed by an increase
in cAMP. Artificially increasing the intracellular concentration of
cAMP by incubation with forskolin or the cAMP analog CPT-cAMP even
resulted in a marked reduction in the rate of acid secretion. The
results demonstrate that primary cell culture provides a useful means
for the analysis of metabolic control and of ion transfer processes
in swimbladder gas gland cells.
Received 1 May 1995; accepted in final form 11 September 1995.
APS Manuscript Number R260-5.
Article publication pending Am. J. Physiol. (Regulatory Integrative
Comp. Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 23 September 1995.