In situ hybridization of h,k-atpase [beta] subunit mrna in rat and
rabbit kidney.
Campbell-Thompson, Martha L., Jill W. Verlander, Kevin A. Curran, W.
Grady Campbell, Brian D. Cain, Charles S. Wingo, and James E.
McGuigan.
Division of Gastroenterology, Hepatology, and Nutrition, Division
of Nephrology, Hypertension, and Transplantation, and Department of
Biochemistry and Molecular Biology, University of Florida College of
Medicine, Gainesville, FL, 32610
APStracts 2:0060F, 1995.
Through a variety of techniques, several investigators have
demonstrated the presence of an H,K-ATPase enzyme in the renal
collecting duct, suggesting that this enzyme serves an important
physiological role in the regulation of acid-base balance and
potassium excretion by the kidney. The present study was designed to
localize cells expressing H,K-ATPase [beta] subunit mRNA in rat and
rabbit kidney by nonradioactive in situ hybridization. A 570-bp DNA
fragment of rabbit renal H,K-ATPase [beta] subunit was used to
produce digoxigenin-labeled riboprobes by in vitro transcription.
Northern blot hybridization demonstrated transcripts in rat gastric
oxyntic mucosa and kidney. In situ hybridization on kidney tissue
sections demonstrated H,K-ATPase [beta] subunit mRNA localization in
epithelial cells, including intercalated cells in the connecting
segment and cortical and medullary collecting duct, principal cells
in the inner stripe of the outer medullary collecting duct, and inner
medullary collecting duct cells in both the rat and the rabbit. These
observations provide evidence that H,K-ATPase [beta] subunit mRNA is
present throughout the collecting duct of the kidney. The
distribution of this message is consistent with a role for H,K-ATPase
in bicarbonate absorption in both the outer and inner medullary
collecting duct.
Received 9 June 1994; accepted in final form 3 April 1995.
APS Manuscript Number F191-4.
Article publication pending Am. J. Physiol. (Renal Fluid Electrolyte
Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 25 April 1995.