Localization of parathyroid hormone/parathyroid hormone- related
peptide receptor mrna in kidney.
Lee, Kaechoong, Dennis Brown, Pablo Ure[tilde]na, Nicole Ardaillou,
Raymond Ardaillou, James Deeds, and Gino V. Segre.
Endocrine Unit, and Renal Unit, Department of Medicine,
Massachusetts General Hospital, and Department of Medicine, Harvard
Medical School, Boston, MA 02114, #INSERM 90, H[circumflex]opital
Necker, Paris, France, and ##INSERM 64, H[circumflex]opital Tenon,
Paris, France
APStracts 2:0138F, 1995.
In kidney, parathyroid hormone (PTH) exerts differential distal
effects along the nephron. In order to define cells expressing
receptors for PTH in kidney, we localized PTH/PTH-related peptide
(PTHrP) receptor mRNA in rat kidney by in situ hybridization.
PTH/PTHrP receptor mRNA is localized to glomerular podocytes,
convoluted and straight proximal tubules, the cortical portion of
thick ascending limbs and distal convoluted tubules, but was not
detected in the thin limb of Henle's loop, or in collecting ducts.
Northern blot analysis showed that cultured human glomerular
podocytes express a unique 4.0 Kb PTH/PTHrP receptor transcript but
do not express detectable levels of the common 2.4 kb transcript
found in whole kidney and in many other tissues. While the tubular
localization of PTH/PTHrP receptor mRNA coincides well with
previously known sites of PTH action, the intense expression and the
unique size of the PTH/PTHrP receptor transcript in glomerular
podocytes suggests that PTH and/or PTHrP may play a role(s) in
glomerular function.
Received 11 May 1995; accepted in final form 31 July 1995.
APS Manuscript Number F152-5.
Article publication pending Am. J. Physiol. (Renal Fluid Electrolyte
Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 14 August 1995.