Na/pi cotransport in flounder: the same transport system in kidney
and intestine.
Kohl, Beate, Peter Herter, Birgit Hlseweh, Marlies Elger, Hartmut
Hentschel, Rolf. K. H. Kinne, and Andreas Werner.
Max-Planck-Institut fr molekulare Physiologie, Rheinlanddamm 201,
44139 Dortmund, Germany, and Mount Desert Island Biological
Laboratory, Salsbury Cove, Maine 04672, Institut fr Anatomie und
Zellphysiologie I, Universitt Heidelberg, Im Neuenheimerfeld 307,
69120 Heidelberg, Germany
APStracts 2:0215F, 1995.
The cloning of a renal Na/Pi cotransport system from winter flounder
(Pseudopleuronectes americanus) has recently been reported. We used
this information to answer the questions: i) what is the distribution
of the transport protein along the nephron, and ii) how are renal and
intestinal transporters related. The distribution of the flounder
NaPi-II protein was tested using two antisera raised against partial
sequences (aa1-aa14, aa388-aa441) of the transporter. Antibody
specific fluorescence was detected at the basolateral membrane of
epithelial cells in the proximal tubular segment PII.
Received 28 August 1995; accepted in final form 28 November 1995.
APS Manuscript Number F285-5.
Article publication pending Am. J. Physiol. (Renal Fluid Electrolyte
Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 12 December 95