Cellular localization of low molecular weight kininogen and
bradykinin b2 receptor mrnas in human kidney.
Song, Qing, Dan-Zhao Wang, Russell A. Harley, Lee Chao, and Julie
Chao.
Department of Biochemistry and Molecular Biology, Department of
Pathology, Medical University of South Carolina, Charleston, South
Carolina 29425, USA
APStracts 2:0191F, 1995.
Kininogen is the precursor of the kinin peptide which binds to kinin
receptors and mediates a broad spectrum of physiological effects. In
order to understand the function of kinin in the kidney, we have
identified the cellular localization of the human low molecular
weight (LMW) kininogen and bradykinin B2 receptor mRNAs in the human
kidney by in situ hybridization histochemistry. Kininogen mRNA was
found in the juxtaglomerular cells, mesangial areas, epithelium of
parietal and visceral (podocytes) layers of Bowman's capsule,
proximal and distal tubules, thin and thick segments of Henle's loop,
collecting ducts and the endothelial cells of the blood vessels. B2
receptor mRNA was co-localized with kininogen mRNA in the kidney
except the podocytes. The most intense signals were observed in the
distal tubules and collecting ducts for both kininogen and B2
receptor mRNAs. No signals were observed in the interstitial cells
and macula densa. Control sections did not stain with either the
kininogen or B2 receptor sense riboprobe. A Northern blot showed that
the expression of LMW kininogen is in the liver and the kidney.
Reverse transcription-polymerase chain reaction Southern blot showed
expression of B2 receptor mRNA in the endothelial cells, renal
proximal tubular cells and kidney. Our results show the sites of
action of kinin in the human kidney, and give further insight into
the physiological role of the kallikrein-kinin system on renal
function.
Received 26 May 1995; accepted in final form 16 October 1995.
APS Manuscript Number F167-5.
Article publication pending Am. J. Physiol. (Renal Fluid Electrolyte
Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 6 November 95