APStracts 3:0107C, 1996.

Effects of osteoclastic resorption on bone surface ion composition. Bushinsky, David A., Konstantin Gavrilov, Victoria M. Stathopoulos, Nancy S. Krieger, Jan M. Chaballa, and Riccardo Levi-Setti. Nephrology Unit, Department of Medicine, University of Rochester, Rochester NY and Enrico Fermi Institute, University of Chicago, Chicago IL

APStracts 3:0107C, 1996.

Osteoclasts are responsible for resorption of bone mineral. The independent effects of osteoclastic resorption on the relative bone mineral ion concentrations of 23Na, 39K and 40Ca have never been determined. To determine how osteoclasts alter bone surface ion composition we isolated neonatal mouse bone cells and cultured them in the presence of PTH (10-9 M) on bovine cortical bone. We compared the surface ion composition of the resulting osteoclastic resorption pits to that of the surrounding unresorbed bone and to neonatal mouse calvariae cultured for 48 h in the presence of PTH utilizing a high resolution scanning ion microprobe.

Received 26 December 1995; accepted in final form 22 March 1996.
APS Manuscript Number C762-5.
Article publication pending Am. J. Physiol. (Cell Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 16 April 96