Cloning and widespread distribution of the rat rod-type cyclic
nucleotide-gated cation channel.
Ding, Changlin, Elizabeth Potter, Weiping Qiu, Steven L. Coon, David
C. Klein, Michael A. Levine, and Sandra Guggino.
Division of Endocrinology and Gastroenterology, Department of
Medicine; and Department of Neuroscience, Johns Hopkins School of
Medicine, 929 Ross Building, 720 Rutland Ave., Baltimore, MD 21205,
Section of Neuroendocrinology, NIH, Bldg. 49, Rm 5A38, 9000 Rockville
Pike, Bethesda, MD 20892
APStracts 3:0384C, 1996.
We used Northern blot analysis, ribonuclease protection assay (RPA),
reverse transcriptase polymerase chain reaction (RT-PCR), and in situ
hybridization to investigate the hypothesis that the CNG1 isoform of
the nucleotide-gated nonselective cation channel may be widely
distributed in tissues of the rat. A cDNA encoding the CNG1 isoform
was isolated from rat eye, human retina and partial sequences from
rat pineal and human kidney. Northern blot analysis revealed 3.1 kb
CNG1 transcript in rat eye, pineal, pituitary, adrenal and spleen,
and a larger transcript of 3.5 kb in testis. RPA confirmed the
identity of CNG1 mRNA in rat eye, lung, spleen and brain. PCR-based
detection of the mRNA for CNG1 indicates that the channel is
expressed in lower abundance in many other tissues, including thymus,
skeletal muscle, heart, and parathyroid gland. The cellular
distribution of CNG1 was further studied by in situ hybridization,
which demonstrated expression of mRNA in lung, thymus, pineal,
hippocampus, cerebellum and cerebral cortex, but not in heart or
kidney.
Received 16 August 1995; accepted in final form 26 November 1996.
APS Manuscript Number C526-5.
Article publication pending Am. J. Physiol. (Cell Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 31 December 1996