Inhibition of glucose transport in human red blood cells by
adenosine antagonists.
Gabel, Scott A., Thomas M. Ooconnell, Elizabeth Murphy, and Robert E.
London.
Laboratory of Molecular Biophysics, NIEHS, Box 12233, Research
Triangle Park, NC 27709
APStracts 3:0398C, 1996.
Previous studies have suggested that adenosine antagonists can
interfere with normal glucose uptake in perfused rat heart. In the
present studies, fluorine-19 NMR spectroscopy was used to study the
effect of the adenosine antagonist, BW-A1433U on the equilibrium
exchange of fluorinated glucose analogs in human erythrocytes.
Studies of the equilibrium exchange of both 2-fluoro-2-deoxy-D
-glucose and 3-fluoro-3-deoxy-D-glucose using either 1D magnetization
transfer and 2D exchange spectroscopy (EXSY) were performed, and
significant inhibition was observed in all cases. Based on
concentration dependent studies, an inhibition constant for the
equilibrium exchange measured at 37 C of 24 uM was determined.
Received 31 July 1996; accepted in final form 22 November 1996.
APS Manuscript Number C429-6.
Article publication pending Am. J. Physiol. (Cell Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 31 December 1996