Ifn-g modulates cd1d surface expression on intestinal
epithelia.
Colgan, Sean P., Victor M. Morales, James L. Madara, John E.
Polischuk, Steven P. Balk, Richard S. Blumberg.
Center for Experimental Therapeutics, Department of Anesthesia and
the Harvard Digestive Diseases Center; Brigham and Women's Hospital
and Harvard Medical School; Division of Gastrointestinal Pathology,
Department of Pathology and the Harvard Digestive Diseases Center;
Brigham and Women's Hospital and Harvard Medical School; Division of
Hematology/Oncology, Beth Israel Hospital and Harvard Medical School;
Division of Gastroenterology, Department of Medicine, Brigham and
Women's Hospital and Harvard Medical School, Boston, Massachusetts
02115
APStracts 3:0047C, 1996.
In vivo, epithelial cells which line the intestine are intimately
associated with lymphocytes, termed intestinal intraepithelial
lymphocytes (iIEL). A putative ligand for iIEL on intestinal
epithelial cells is CD1d, and recent studies demonstrate a surface
form of this molecule exists on intestinal epithelia. At present, it
is not known whether CD1d expression is regulated by cytokines in the
intestinal microenvironment. Thus, we examined the impact of relevant
cytokines on CD1d at the level of mRNA and cell surface expression.
Using a sensitive whole cell ELISA, we assessed the impact of
relevant cytokines on CD1d expression on intestinal epithelial cell
lines. We were readily able to detect CD1d on the surface of T84
cells, a crypt-like intestinal epithelial cell line. Epithelial cell
exposure to human recombinant interferon-g (IFN-g) resulted in
increased CD1d expression in a dose and time-dependent manner. PCR
amplification of CD1d cDNA revealed a time-dependent induction
following exposure to IFN-g. This IFN-g effect on CD1d expression was
cytokine-specific and was evident using epithelial cell lines other
than T84, including CaCO2 and HT29 cells. Finally, we were not able
to detect significant surface expression of CD1a, CD1b or CD1c on
intestinal epithelial cell lines in the presence or absence of
relevant cytokines. These results indicate that CD1d cell surface
protein and cellular mRNA, like other MHC-related molecules, is
cytokine regulated in intestinal epithelial cell lines.
Received 6 December 1995; accepted in final form 24 January 1996.
APS Manuscript Number C735-5.
Article publication pending Am. J. Physiol. (Cell Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 8 February 96