T cell lymphokines modulate fibroblast growth factor-induced smooth
muscle cell fibrinolysis and migration.
Wang, Weizheng, Hong Jun Chen, Allan Schwartz, Paul J. Cannon, Leroy
E. Rabbani.
Division of Cardiology, Department of Medicine, Columbia University
College of Physicians and Surgeons, New York, NY 10032
APStracts 3:0277C, 1996.
Smooth muscle cell (SMC) fibrinolysis is necessary for SMC migration.
To determine whether the T cell lymphokines interleukin-4 (IL-4) and
gamma interferon (IFN-[gamma]) modulate SMC fibrinolysis and
migration induced by basic fibroblast growth factor (bFGF), we
examined the effects of IL-4 and IFN-[gamma] on human SMC tissue-type
plasminogen activator (TPA), urokinase-type plasminogen activator
(UPA), and plasminogen activator inhibitor-1 (PAI-1) antigen
production, determined by enzyme-linked immunosorbent assays.
Although IL-4 had no effects on SMC TPA, UPA, and PAI-1 production,
it potentiated bFGF-induced TPA, UPA, and PAI-1 antigens. IL-4 plus
bFGF resulted in a net increase in SMC fibrinolytic activity. IFN
-[gamma] did not significantly affect bFGF induction of SMC TPA and
PAI-1 antigens. However, IFN-[gamma] significantly decreased bFGF
-mediated induction of SMC UPA antigen. IFN-[gamma] decreased the IL-4
plus bFGF induction of both TPA and UPA antigens. IL-4 increased and
IFN-[gamma] abrogated bFGF induction of in vitro SMC migration
through a modified micro-Boyden chamber. Therefore, IL-4 and IFN
-[gamma] modulate bFGF-mediated induction of in vitro vascular SMC
fibrinolysis and migration.
Received 10 May 1996; accepted in final form 7 August 1996.
APS Manuscript Number C251-6.
Article publication pending Am. J. Physiol. (Cell Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 19 September 1996