Microdialysis measurements in skin: evidence for significant
lactate release in healthy man.
Jansson, Per-Anders, Anne Lene Krogstad, and Peter
L[diaeresis]onnroth.
The Lundberg Laboratory for Diabetes Research, Department of
Internal Medicine and Department of Dermatology, Sahlgrenska
University Hospital, Gothenburg, Sweden
APStracts 3:0070E, 1996.
To assess net lactate release from dermal skin, seven lean healthy men
were studied after fasting overnight. Two microdialysis catheters
were inserted in the upper dermal tissue, as ensured by ultrasound
scanning, in the periumbilical area. Each catheter was calibrated in
situ to get an estimate of the lactate concentration in interstitial
fluid (1001 +/- 24 [mu]mol/l), which in turn enabled calculation of
the local capillary-venous lactate concentration (963 +/- 25
[mu]mol/l). Concomitantly, arterialized venous plasma lactate (673
+/- 32 [mu]mol/l), blood hematocrit (43 +/- 1%) and skin blood flow
(3.8 +/- 0.9 ml/100g/min) as measured by 133Xe-clearance were
determined and dermal apparent lactate release (570 +/- 89
nmol/100g/min) estimated according to Fick. During an oral glucose
tolerance test (OGTT, 75 g) the dermal interstitial-arterial lactate
difference decreased significantly to reach a nadir value at 60 min.
Moreover, no significant increase in skin blood flow was seen during
the OGTT. In conclusion, we found a significant net lactate release
from abdominal dermal skin after fasting overnight whereas no
significant increase was observed during an OGTT.
Received 3 October 1995; accepted in final form 28 February 1996.
APS Manuscript Number E486-5.
Article publication pending Am. J. Physiol. (Endocrinol. Metab.).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 1 April 96