Cobalt but not hypoxia stimulates pdgf-gene expression in rats
.
Bucher, Michael, Peter Sandner, Konrad Wolf, and Armin Kurtz.
Institut f[umlaut]ur Physiologie der Universit[umlaut]at
Regensburg, D-93040 Regensburg, Germany
APStracts 3:0079E, 1996.
This study was done to investigate the influence of different forms of
acute tissue hypoxia on the expression of PDGF-A and PDGF-B chain
genes in different rat organs. We found that acute normobaric hypoxia
(8% O2), carbon monoxide inhalation (0.1% CO) or lowering the
hematocrit to 12% for 6 hours had no effect on PDGF-A or PDGF-B gene
expression in lung, heart, kidney and liver of Sprague Dawley rats.
Subcutaneous administration of cobaltous chloride dose dependent
increased PDGF-B mRNA by 125% in lungs by 60% in kidneys but not in
heart and liver. These findings suggest that acute tissue
hypoxygenation is not a significant stimulus for PDGF-A and PDGF-B
gene expression in these major rat organs. Cobalt appears to cause a
tissue specific increase of PDGF-B gene expression.
Received 19 December 1995; accepted in final form 28 March 1996.
APS Manuscript Number E593-5.
Article publication pending Am. J. Physiol. (Endocrinol. Metab.).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 23 April 96