Measurement of the interstitial muscle glucose and lactate
concentrations during an oral glucose tolerance test.
M., M[umlaut]uller, Holm[umlaut]ang A., Andersson O. K., Eichler H.
G., L[diaeresis]onnroth P.
Lundberg Laboratory for Diabetes Research, Sahlgrenska University
Hospital, Sweden and Department of Clinical Pharmacology, University
of Vienna, Austria Key words: Skeletal muscle, glucose metabolism,
microdialysis, muscle blood flow
APStracts 3:0154E, 1996.
To study the relationship between the blood flow rate and the muscle
metabolism muscle microdialysis was performed in nine human subjects
(F/M = 5/4) after an oral glucose load (75 g). Two microdialysis
probes were inserted in the medial femoral muscle for estimation of
glucose and lactate concentrations in the interstitial fluid, and the
muscle blood flow was measured concomitantly with strain-gauge
plethysmography. After fasting over night glucose concentration in
arterial plasma and interstitial fluid was 4.6+/-0.13 vs 3.8+/-0.23
mmol/l (p<0.05) and the corresponding lactate concentrations
0.60+/-0.07 vs 0.83+/-0.07 mmol/l (p<0.05). Muscle blood flow
was 5.2+/-0.7 and 7.5+/-1.4 ml x 100 g-1 x min-1 (p<0.05) at 0
and 90 min after oral glucose, respectively. The arterial
-interstitial concentration differences of glucose increased after
oral glucose (at 0' 0.73+/-0.24 vs 2.19+/-0.60 mmol/l at 90' (p<
0.001) The corresponding values for lactate were -0.23+/-0.10 at 0'
vs -0.26+/-0.18 mmol/l at 90', (n. s.). The data show that 1) the
capillary wall is partly rate limiting for glucose uptake and 2)
after oral glucose the glucose concentration gradient over the
capillary wall increases despite a limited increase in blood flow
rate which, then, mediates approximately 10-20% of total enhancement
of glucose uptake in muscle.
Received 27 March 1996; accepted in final form 23 July 1996.
APS Manuscript Number E151-6.
Article publication pending Am. J. Physiol. (Endocrinol. Metab.).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 21 August 1996