Reciprocal glut-1 and glut-4 expression and glucose transport in
denervated muscles.
Handberg, A., L. A. Megeney, K. J. A. McCullagh, L. Kayser, X-X. Han,
A. Bonen.
DEPARTMENT OF MEDICAL PHYSIOLOGY AND ANATOMY, PANUM INSTITUTE,
COPENHAGEN, DENMARK, DEPARTMENT OF KINESIOLOGY, UNIVERSITY OF
WATERLOO, WATERLOO, ONTARIO, CANADA
APStracts 3:0032E, 1996.
We investigated in 3 day denervated muscles a) the expression of GLUT
-1 in perineurial sheaths (PNS) and muscle, b) the muscle fiber
specific changes in GLUT-1 and GLUT-4, and c) changes in basal and
insulin-stimulated 3-O-methyl glucose (3-O-MG) transport. GLUT-1 was
increased in both the PNS (P<0.05) and in the muscle membranes
(P<0.05). GLUT-1 and GLUT-4 concentrations were changed
reciprocally, in a fiber dependent fashion (GLUT-1: RG, +31%; WG,
+10%; GLUT-4: RG, -53%; WG, -16%). Basal glucose transport was
increased (P<0.05) and this increase was correlated with the
oxidative nature of the muscles (r =0.97). Insulin-stimulated glucose
transport was decreased in denervated muscles (P<0.05). This was
also related to the oxidative nature of the muscles (r = -0.88). The
increase in basal glucose transport was correlated with the loss of
insulin-stimulated transport (r = 0.95). Thus, the increase in GLUT-1
compensates for the loss of GLUT-4, resulting in a 56% regain of the
reduced, insulin-stimulated glucose transport.
Received 13 June 1995; accepted in final form 22 January 1996.
APS Manuscript Number E272-5.
Article publication pending Am. J. Physiol. (Endocrinol. Metab.).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 8 February 96