Paracrine stimulation of preadipocyte-enriched cell cultures by
mature adipocytes.
Considine, Robert V., Mark R. Nyce, Luz Marina Morales, Susan A.
Magosin, Madhur K. Sinha, Thomas L. Bauer, Ernest L. Rosato, James
Colberg, and Jose F. Caro.
Division of Endocrinology and Metabolism, Department of Medicine,
and Department of Surgery, Jefferson Medical College of Thomas
Jefferson University, Philadelphia, Pennsylvania 19107
APStracts 3:0033E, 1996.
We have developed a double chamber system in which to examine the
effects of mature adipocytes on the growth and differentiation of
preadipocytes and other cells in the adipose tissue. In the present
study we have found that mature adipocytes from both lean and obese
subjects release a factor that stimulates the growth of preadipocyte
-enriched and dedifferentiated adipocyte-enriched cell cultures. This
growth stimulation was dependent on both time of exposure to mature
cells and the number of mature cells in the coculture. Proliferation
of the preadipocyte-enriched (n=4) and dedifferentiated adipocyte
-enriched cultures (n=5) in the presence of mature adipocytes from
obese subjects (BMI> 35) was 4.1 and 2.9 fold more (p<0.05)
than that in the presence of adipocytes from lean subjects (BMI<
25). There was no difference in the growth of cultures enriched in
preadipocytes or dedifferentiated adipocytes from lean or obese
subjects in the absence of mature adipocytes. These observations
demonstrate that mature adipocytes from obese patients stimulate the
growth of preadipocyte-enriched cultures to a greater extent than
those from lean individuals.
Received 1 September 1995; accepted in final form 5 January 1996.
APS Manuscript Number E129-5.
Article publication pending Am. J. Physiol. (Endocrinol. Metab.).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 14 February 96