Aluminum potentiates pi transport stimulation induced by fluoride in osteoblast-like cells. Imai, Toshio, Dominique Burgener, Xuechu Zhen, Jean-Philippe Bonjour, and Joseph Caverzasio. Division of Clinical Pathophysiology, Department of Medicine, University Hospital of Geneva, CH - 1211 Geneva 14, Switzerland
APStracts 3:0127E, 1996.
The effect of aluminum (Al) on inorganic phosphate (Pi) transport stimulation induced by fluoride (F) was investigated in MC3T3-E1 osteoblast-like cells. Al dose- and time-dependently potentiated the increase in Pi transport activity induced by F. Rsults obtained with deferoxamine mesylate, a Al-chelator suggests that a fluoroalumino complex is probably the active F molecule responsible for the change in Pi transport observed in this study. The signaling pathway responsible for the stimulation of Pi transport by F+Al likely involves a tyrosine phosphorylation process but neither a protein kinase C nor a MAP kinase pathway. As previously found in UMR-106 cells for F alone, F+Al potentiated the change in Pi transport induced by foetal calf serum. A similar interaction was found between F+Al and thrombin acting through a G-protein coupled receptor. These observations are compatible with the hypothesis that F+Al could interact with G-protein-coupled receptors associated with a signaling tyrosine phosphorylation process involved in the regulation of Pi transport in osteoblast-like cells. 

Received 26 January 1996; accepted in final form 4 June 1996.
APS Manuscript Number E47-6.
Article publication pending Am. J. Physiol. (Endocrinol. Metab.).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 25 July 1996