Pig pancreatic acinar cells possess predominantly the cckb
cholecystokinin receptor subtype.
Morisset, Jean, Fran[cedilla]coise Levenez*, Tristan Corring, Ouhida
Benrezzak, Ghislain Pelletier, and Ezequiel Calvo.
D[acute]epartement de m[acute]edecine, Facult[acute]e de
m[acute]edecine, Universit[acute]e de Sherbrooke, Sherbrooke,
Qu[acute]ebec, Canada J1H 5N4, and INRA, Jouy en Josas, France
APStracts 3:0108E, 1996.
Studies performed on pig indicated that its pancreas was insensitive
to the gastrointestinal hormone cholecystokinin (CCK) and suggested
that its secretions were rather under the control of the
neurotransmitter acetylcholine. This study was performed to determine
reasons for this insensitivity by comparing secretory responses to
different secretagogues and establishing the dominant CCK receptor
type. Pancreatic acini prepared from weaned piglets were evaluated
for their sensitivity to carbamylcholine (Cch), caerulein, JMV-180
and secretin. RNAs were extracted for CCKA and CCKB receptors'
expression using specific cRNA probes. Results indicate that pig
pancreatic acini are sensitive to Cch, relatively insensitive to
caerulein with no response to JMV-180, a CCKA agonist, nor secretin;
MK329, a CCKA receptor antagonist, significantly inhibited caerulein
-induced enzyme secretion from 10-6M. The pig pancreas expresses few
CCKA mRNA receptors but a majority of CCKB. These data demonstrate
that the pig pancreas expresses a majority of CCKB receptors. In
conclusion, the pig pancreas possesses a large majority of CCKB
receptors responsible for their low sensitivity to CCK.
Received 2 March 1995; accepted in final form 18 May 1995.
APS Manuscript Number E102-5.
Article publication pending Am. J. Physiol. (Endocrinol. Metab.).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 17 June 96