Kinetic aspects of acetate metabolism in healthy humans using [ 1
-13 c]acetate.
Pouteau, E., H. Piloquet, P. Maugeais, M. Champ, H. Dumon, P. Nguyen,
M. Krempf.
Laboratory of Human Nutrition, H[circumflex]otel Dieu, Nantes,
Laboratory of Animal Nutrition, Veterinary School, Nantes, National
Institute for Agricultural Research, Nantes
APStracts 3:0052E, 1996.
Acetate metabolism in humans is not well known. Kinetic aspects of
acetate were investigated in the post absorptive state on healthy
subjects. In a first study, six subjects were infused with a prime
constant infusion of [1-13C] acetate for 3h and a prime of NaH13CO3.
No difference was found between arterialized and venous tracer
enrichments from the arm, while arterialized acetate concentrations
were higher (74 +/- 12 vs 59 +/- 14 [mu]mol.l-1, p < 0.05)
suggesting that the hand muscles used but did not produce acetate in
the post absorptive state. Total body flux of acetate was 8.4 +/- 0.6
[mu]mol.kg-1.min-1 of which 69 +/- 5 % were oxidized. Acetate
contributed to 6.5 +/- 0.4 % of the basal energy expenditure. In a
second study, five volunteers were submitted to a gastric infusion
for 3h followed by an intravenous infusion for 3h of [1-13C] acetate.
Higher fluxes were observed with the tracer gastric infusion and the
first pass removal of acetate within the splanchnic bed was 60 +/- 7
%. Acetate contributes significantly to the energy supply of the
body. It is mainly used by the liver when produced in the gut.
Received 27 September 1995; accepted in final form 19 February
1996.
APS Manuscript Number E472-5.
Article publication pending Am. J. Physiol. (Endocrinol. Metab.).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 20 March 96