Levels of plasma c19 steroids and 5[alpha]-reduced c19 steroid
glucuronides in primates, rodents and domestic animals.
Guillemette, Chantal, Dean W. Hum, and Alain B[acute]elanger.
MRC Group in Molecular Endocrinology, CHUL Research Center and
Laval University, Quebec, G1V 4G2, Canada
APStracts 3:0086E, 1996.
In this study, a comparison of circulating levels of ADT-G and
3[alpha]-DIOL-G in the male and female of several mammalian species
was performed. Glucuronidated steroids were not detected in the
circulation of the dog, bovine, porcine and rodent. High levels of
circulating glucuronidated steroids were measured in Cynomolgus
monkey and were 10-fold higher than in human. The determination of
tissue levels of unconjugated and conjugated C19steroids was then
performed in intact and castrated rats treated with androgens.
Steroid glucuronides were not detected in the plasma, skin, prostate
and liver of both intact and treated rats although the levels of
unconjugated steroids in the plasma and tissues were increased
following steroid treatments. Significant levels were detected in the
bile thus suggesting hepatic formation of steroid glucuronides in the
rat. It is suggested that the monkey represents the best animal model
to date to study the contribution of UGTs, present in steroid target
peripheral tissues, to circulating levels of steroid glucuronides.
Received 18 October 1995; accepted in final form 1 April 1996.
APS Manuscript Number E500-6.
Article publication pending Am. J. Physiol. (Endocrinol. Metab.).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 1 May 96