Nutritional status affects branched-chain oxo acid dehydrogenase
activity during exercise in humans.
Jackman, Michelle L., Martin J. Gibala, Eric Hultman, and Terry E.
Graham.
Department of Human Biology and Nutritional Sciences, University of
Guelph, Guelph, Ontario, N1G 2W1, CANADA and Department of Clinical
Chemistry, Huddinge University Hospital, Karolinska Institute, S-141
86, Huddinge, SWEDEN
APStracts 3:0203E, 1996.
We examined the effect of glycogen availability and branched-chain
amino acid (BCAA) supplementation on branched-chain oxo acid
dehydrogenase (BCOAD) activity during exercise. Six subjects cycled
at 75% of their maximal oxygen uptake to exhaustion on 3 occasions
under different pre-exercise conditions: (1) low muscle glycogen
(LOW), (2) low muscle glycogen plus BCAA supplementation (LOW+BCAA),
and (3) high muscle glycogen. The LOW trial was performed first,
followed by the other 2 conditions in random order, and biopsies for
all trials were obtained at rest, after 15 min of exercise (15Min)
and at the point of exhaustion during the LOW trial (49Min). BCOAD
activity was not different among the 3 conditions at rest, however at
15Min BCOAD activity was higher (P
Received 13 August 1996; accepted in final form 1 October 1996.
APS Manuscript Number E398-6.
Article publication pending Am. J. Physiol. (Endocrinol. Metab.).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 5 November 1996