Ileal transposition into the upper jejunum affects lipid and bile
salt absorption in rats.
Tsuchiya, Takashi, Theodore J. Kalogeris, and Patrick Tso.
Department of Physiology and Biophysics, Louisiana State University
Medical Center, Shreveport, LA 71130
APStracts 3:0067G, 1996.
In order to determine whether ileal transposition affects absorption
and transport of lipids and bile salts, we studied the absorption,
and lymphatic transport of triglyceride, cholesterol, and sodium
taurocholate in rats with the distal quarter of their small bowel
transposed to the proximal jejunum and in control rats whose
intestines were transected and reanastomosed without transposition.
Three weeks after transposition or sham surgery, rats were equipped
with duodenal or jejunal and intestinal lymph duct cannulas. They
were then given continuous duodenal or jejunal infusions of lipid
emulsion containing triolein (40 [mu]mol/h + 3H-triolein) and
cholesterol (7.8 [mu]mol/h + 14C-cholesterol) for 8 h. Lymph lipid
output was measured; after 8 h of lipid infusion, luminal and mucosal
radioactive lipid distribution was also quantified. Transposition had
no effect on triglyceride absorption and transport, but cholesterol
absorption and transport were both significantly attenuated in the
transposed rats. In a separate study we examined whether ileal
transposition would alter the kinetics of bile salt absorption. Six
weeks after either transposition or sham surgery, rats were given a
duodenal bolus injection of 14C-sodium taurocholate mixed in rat
bile, and the output of radiolabeled bile salt through a bile fistula
was measured. Appearance of radiolabelled taurocholate was gradual in
the control rats, peaking at about 90 min after administration.
Appearance of labelled bile salt was rapid in the transposed rats,
peaking within 60 min after administration. In conclusion, ileal
transposition has no effect on triglyceride absorption, but
attenuates cholesterol absorption and transport, possibly by
promoting premature absorption of bile salts.
Received 24 July 1995; accepted in final form 22 March 1996.
APS Manuscript Number G314-5.
Article publication pending Am. J. Physiol. (Gastrointest. Liver
Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 1 April 96