The effect of gastric inhibitory polypeptide and glucagon like
peptides on intestinal basolateral membrane hexose transport.
Cheeseman, Chris I, and Raymond Tsang.
Dept. of Physiology, University of Alberta, Edmonton, Alta, T6G
2H7
APStracts 3:0071G, 1996.
The effect of gastric inhibitory polypeptide (GIP) and the related
glucagon like peptides 1 and 2 (GLP-1 and GLP-2) on jejunal
basolateral membrane glucose transport was investigated to determine
if the up-regulation produced by luminal hexoses could be explained
by the release of one or more of these peptides. Luminal perfusion of
the rat jejunum for four hours, under sodium pentabarbital
anesthesia, with 100mM D-glucose produced a significant increase in
plasma GIP levels. Vascular infusion of saline containing 100 - 800pM
GIP also increased the maximal transport rate for carrier mediated
glucose uptake in jejunal basolateral membrane vesicles. The effect
of vascular 400 pM GIP was maximal after one hour and maintained out
to four hours. The effect of luminal glucose could be blocked by
preinjection with anti-GIP antibodies, while an anti-neurotensin
antibody had no effect. Vascular infusion with 800 pM GLP-1 (7-36)
amide had no effect, but GLP-2 (400 & 800pM) increased the D
-glucose maximal transport rate. An anti-GLP antibody was able to
block the response to luminal glucose.
Received 28 February 1995; accepted in final form 4 March 1996.
APS Manuscript Number G91-5.
Article publication pending Am. J. Physiol. (Gastrointest. Liver
Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 16 April 96