Detection of nitric oxide production and its role in pancreas
ischemia/reperfusion in rats.
Tanaka, Satonori, Wataru Kamiike, Hiroaki Kosaka, Toshinori Ito, Eiji
Kumura, Takeshi Shiga, and Hikaru Matsuda.
First Department of Surgery and First Department of Physiology,
Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565,
Japan
APStracts 3:0072G, 1996.
Partial ischemia of the rat pancreas body and tail was obtained by
occlusion of the celiac axis for 1 hr after gastrectomy. The plasma
level of nitrite plus nitrate in both the systemic and portal venous
blood after reperfusion was significantly higher than that after sham
operation and ischemia alone. The elevation after reperfusion was
significantly decreased by NG-nitro-L-arginine methyl ester (L-NAME).
Simultaneous administration of L-arginine counteracted the L-NAME
-induced decrease in the level of NO end products. Generation of NO
was further demonstrated by nitrosylhemoglobin detection by electron
spin resonance in the blood after reperfuson. On the other hand, the
plasma level of lipase, a marker of damage to pancreatic exocrine
tissue, was significantly increased after ischemia/reperfusion, and
it was further increased by administration of L-NAME. This increase
in the lipase correlated with a decrease in the tissue blood flow in
the pancreas. These results suggest that NO is generated during
ischemia/reperfusion of the rat pancreas and NO may have a protective
role in ischemic /reperfusion of the pancreas.
Received 2 October 1995; accepted in final form 15 March 1996.
APS Manuscript Number G425-5.
Article publication pending Am. J. Physiol. (Gastrointest. Liver
Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 16 April 96