Vasoactive intestinal peptide modulates t lymphocyte migration in
peyer's patches of rat small intestine.
Miura, Soichiro, Hiroshi Serizawa, Yoshikazu Tsuzuki, Iwao Kurose,
Makoto Suematsu, Hajime Higuchi, Takeharu Shigematsu, Ryota Hokari,
Masahiko Hirokawa, Hiroyuki Kimura, and Hiromasa Ishii.
Departments of Internal Medicine and Biochemistry, School of
Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo 160,
Japan
APStracts 3:0155G, 1996.
Although vasoactive intestinal peptide has been postulated to function
in modulation of T-cell trafficking, the exact mechanism has not been
elucidated in vivo. In the present study, the effects of vasoactive
intestinal peptide on T-lymphocyte migration were examined in rat
Peyer's patches. T-lymphocytes collected from intestinal lymph of
rats were labeled with carboxyfluorescein diacetate succinimidyl
ester, and injected into the jugular vein. Peyer's patches of the
recipient rats were observed with intravital fluorescence microscopy.
In vivo intraarterial infusion of or in vitro incubation with
vasoactive intestinal peptide did not affect the initial lymphocyte
interaction with postcapillary venules of Peyer's patches. However,
these treatments with vasoactive intestinal peptide significantly
inhibited transendothelial migration and also significantly blocked
the interstitial migration of T-cells and inhibited their subsequent
appearance in the interfollicular lymphatics. Treatment with cyclic
AMP inducing agents resulted in similar inhibitory effects on T
-lymphocyte migration in Peyer's patches. In conclusion, vasoactive
intestinal peptide has significant inhibitory effects on T-lymphocyte
migration in Peyer's patches, possibly mediated by the elevation of
the intracellular cyclic AMP concentrations.
Received 25 January 1996; accepted in final form 26 July 1996.
APS Manuscript Number G38-6.
Article publication pending Am. J. Physiol. (Gastrointest. Liver
Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 29 August 1996