Cytokine regulation of fibroblast growth factor receptor 3 iiib in
intestinal epithelial cells.
Kanai, Michiyuki, Ian Rosenberg, and Daniel K. Podolsky.
Gastrointestinal Unit and Center for the Study of Inflammatory
Bowel Disease, Department of Medicine, Massachusetts General Hospital
and Harvard Medical School. Boston, MA 02114
APStracts 3:0252G, 1996.
Proliferation and function of the intestinal epithelium is modulated
by a range of regulatory peptides including cytokines and peptide
growth factors. In order to define mechanisms integrating these
regulatory systems, the effects of growth factors and cytokines on
the expression of the fibroblast growth factor receptor 3 IIIb
expressed on intestinal epithelial cells were examined in Caco-2
cells. Regulated expression of FGFR3 IIIb was associated with
acquisition of the differentiated state. Keratinocyte growth factor
(KGF), a ligand of another member of the FGFR family enhanced
expression of FGFR3 IIIb, while acidic FGF (aFGF), ligand for FGFR3
IIIb itself, had no effect. Epidermal growth factor (EGF) and
transforming growth factor b (TGFb) also markedly enhanced FGFR3 IIIb
expression in a different temporal pattern. In addition, FGFR3 IIIb
expression was increased 10-fold by the cytokine interleukin-2 (IL
-2). These studies demonstrate integration between cytokines and
growth factor ligand-receptor systems in intestinal epithelial cells.
Received 16 January 1996; accepted in final form 25 October 1996.
APS Manuscript Number G22-6.
Article publication pending Am. J. Physiol. (Gastrointest. Liver
Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 31 December 1996