Cellular localization of mrnas for the prostaglandin e receptor
subtypes in the mouse gastrointestinal tract.
Morimoto, Kimiko, Yukihiko Sugimoto, Masato Katsuyama, Hiroji Oida,
Kazuhito Tsuboi, Kiyohiko Kishi,yoshikazu Kinoshita, Manabu Negishi,
Tsutomu Chiba, Shuh Narumiya, and Atsushi Ichikawa.
Department of Physiological Chemistry, Faculty of Pharmaceutical
Sciences, Department of Pharmacology, Faculty of Medicine, Division
of Gastroenterology and Hepatology, Department of Medicine,
Postgraduate School of Medicine, Kyoto university, Kyoto 606, and
Division of Gerontology, Department of Medicine, Kobe University
School of Medicine, Kobe 650, Japan
APStracts 3:0260G, 1996.
Regional and cellular distribution of mRNAs for the prostaglandin E
(PGE) receptor subtypes was investigated in the mouse
gastrointestinal tract by in situ hybridization. Strong signals for
EP1 transcripts were detected in the cells of the muscularis mucosae
layer, especially in the body of the stomach. Intense signals for EP3
transcripts were detected in the neurons of the myenteric ganglia
throughout the tract. Moderate EP3 mRNA expression was also observed
in fundic gland epithelial cells, except for the surface mucous
cells, in the stomach. Expression of EP4 mRNA was moderate in the
surface epithelial cells of the corpus, and in the glands from the
surface to the base of the antrum. Strong EP4 signals were observed
in the epithelium in the duodenum, jejunum, and ileum. In the ileum,
signals were only observed in the upper part of the villi. However,
no or weak signals for EP2 transcripts were detected. These findings
suggest that PGE2 modulates various gastric or intestinal functions
via at least three different PGE receptors.
Received 6 August 1996; accepted in final form 13 November 1996.
APS Manuscript Number G321-6.
Article publication pending Am. J. Physiol. (Gastrointest. Liver
Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 31 December 1996