Taurocholate induces directional excretion of bilirubin into biles
in the perfused rat liver.
Yamaguchi, Tokio, Yoshiyuki Wakabayashi, Makiko Tanaka, Tsuyoshi Sano,
Hiromi Ishikawa, Hiroshi Nakajima, Makoto Suematsu, Yuzuru Ishimura.
Department of Biochemical Genetics, Medical Research Institute,
Tokyo Medical and Dental University, Tokyo 113, Department of
Biochemistry, School of Medicine, Keio University, Tokyo 160,
Japan
APStracts 3:0034G, 1996.
The role of taurocholate, one of the major conjugated bile acids
present in portal blood in excretion of bilirubin from liver
parenchyma to biliary and vascular compartments was studied in
isolated perfused rat liver. Contents of bilirubin and carbon
monoxide (CO) in the bile or venous effluents were determined by an
enzyme-linked immunosorbent microassay using an anti-bilirubin
monoclonal antibody 24G7 and myoglobin-assisted spectrophotometry,
respectively. In the presence of taurocholate, bilirubin excreted
into the biliary compartment constituted greater than 90% of the
total bilirubin excreted from the liver (0.26 nmol / min / g liver)
corresponding to 60% of the outflow of CO into the venous effluents.
In its absence, however, total amount of bilirubin excreted into
extrahepatic compartments was reduced to 27% of the CO flux, and more
than 90% of the excreted bilirubin was in the venous effluent. Thus,
a choleretic bile acid such as taurocholte is necessary for
directional transport of bilirubin into biles in the perfused liver
preparation.
Received 30 October 1995; accepted in final form 25 January 1996.
APS Manuscript Number G470-5.
Article publication pending Am. J. Physiol. (Gastrointest. Liver
Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 8 February 96