Immunoreactive interleukin-1[beta] release from the rat gastric fundus is reduced by glucocorticoids and inhibitors of gastric acid secretion. Montuschi, Paolo, Giuseppe Tringali, Adriana Mirtella, Luca Parente, Enzo Ragazzoni, Paolo Preziosi, and Pierluigi Navarra. Institutes of Pharmacology, School of Pharmacy, University of Palermo, via Forlanini 1 - 90134 Palermo, and Catholic University Medical School, Largo Francesco Vito 1 - 00168 Rome, Italy
APStracts 3:0021G, 1996.
Interleukin-1 (IL-1) is known to regulate gastric functions via a central action at the hypothalamic level, and it has also been shown that this cytokine can directly modulate rat gastric motility. This study was conducted to determine whether IL-1[beta] is produced and released by rat gastric fundi in vitro. IL-1[beta] immunoreactivity was released in measurable amounts from explanted rat gastric tissue. This release was not affected by electrical stimulation of the gastric strips or by agents that induce IL-1 biosynthesis. It could be inhibited only by the glucocorticoid, dexamethasone. Ex-vivo experiments confirmed the inhibitory role of glucocorticoids and showed that IL-1[beta] release can be inhibited by agents that reduce gastric acid secretion, suggesting that the latter might stimulate IL-1[beta] synthesis and release. In light of the well established gastroprotection exerted by IL-1, H+-induced IL-1[beta] release might serve as a protection against mucosal injuries caused by acid secretion, and the inhibition of this release by glucocorticoids might be involved in the pathogenesis of gastric damage associated with severe stress or steroid therapy.

Received 9 March 1995; accepted in final form 16 January 1996.
APS Manuscript Number G103-5.
Article publication pending Am. J. Physiol. (Gastrointest. Liver
Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 29 January 96