Cloning and analysis of cdnas differentially expressed in the
adapting remnant intestine after partial small bowel resection.
Dodson, Brian D., Joseph L. Wang, Elzbieta A. Swietlicki, Deborah C.
Rubin, and Marc S. Levin.
Department of Medicine, Washington University School of Medicine.,
St. Louis, MO
APStracts 3:0023G, 1996.
Following partial resection, the remnant small intestine undergoes an
adaptive response. Little is known about the molecular and cellular
basis of intestinal adaptation. To identify genes transcriptionally
regulated in response to loss of functional bowel surface area, we
have isolated cDNAs differentially expressed in the adaptive ileum 48
h after 70% proximal small intestinal resection. A cDNA library
constructed from the remnant ileum of rats subjected to resection was
screened using subtractive hybridization techniques. Several groups
of cDNA clones that were induced during intestinal adaptation were
isolated. The first included liver fatty acid binding protein,
apolipoprotein AIV, cellular retinol binding protein II, and ileal
lipid binding protein. These all encode proteins involved in the
absorption, metabolism, and trafficking of nutrients. A second group
included the catalytic subunit of protein phosphatase 1d, GRP 78, a
glucose regulated member of the HSP70 family of heat shock proteins,
and several pancreatitis associated proteins. A third group of
induced genes contained novel cDNAs. To better characterize the
adaptive response, the temporal, spatial and cellular patterns of
expression of several of these genes were analyzed using
immunohistochemical and in situ hybridization techniques. These
studies indicate during early adaptation, genes involved in nutrient
trafficking, protein processing, and cell cycle regulation are
transcriptionally regulated in the residual small intestine in
distinct temporal and regional patterns consistent with a complex
multifaceted response to intestinal resection.
Received 5 September 1995; accepted in final form 12 January
1996.
APS Manuscript Number G393-5.
Article publication pending Am. J. Physiol. (Gastrointest. Liver
Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 29 January 96