The effect of cholecystokinin and related peptides on jejunal
transepithelial hexose transport in the sprague-dawley rat.
Hirsh, Andrew J., Raymond Tsang, Srinu Kammila, and Chris I.
Cheeseman.
University of Alberta, Department of Physiology, 7-55 Medical
Sciences Building, Edmonton, Alberta, CANADA, T6G 2H7
APStracts 3:0119G, 1996.
An in situ dual vascular and luminal perfusion technique was used to
study the effect of CCK-8 on the transport of hexoses by the jejunum
of the Sprague-Dawley rat from the lumen to the vascular bed. The
lumen of the jejunum was perfused with hexoses in oxygenated Krebs
buffer, while the superior mesenteric artery was infused with Krebs
buffer containing Ficoll 70 as a plasma expander. CCK-8 (0.8-8 pM) in
the vascular infusate selectively reduced hexose transport in a dose
-dependent manner by 20 to 47 % while having no effect on L-glucose or
L-leucine absorption. Vascular tetrodotoxin did not block the CCK-8
inhibition, whereas, a specific CCK-A receptor antagonist,
lorglumide, did. The CCK-B receptor agonist, cholecystokinin
-tetrapeptide, had a small effect on hexose absorption while
somatostatin 14 and 28 had no effect. These results suggest that
cholecystokinin can decrease intestinal absorption of hexoses in the
small intestine, acting via CCK-A type receptors.
Received 22 September 1995; accepted in final form 18 April 1996.
APS Manuscript Number G417-5.
Article publication pending Am. J. Physiol. (Gastrointest. Liver
Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 28 June 96