Phospholipase a2 stimulation of rat intestinal epithelial cell (
iec-6) migration.
Minami, Takeshi, Shinichiro Zushi, Yasuhisa Shinomura, Yuji Matsuzawa.
The Second Department of Internal Medicine, Osaka University
Medical School, 2-2 Yamadaoka, Suita, Osaka 565, Japan
APStracts 3:0098G, 1996.
The effect of phospholipase A2 (PLA2) on intestinal epithelial cell
migration was investigated using an in vitro wounding model of
confluent monolayers of IEC-6. PLA2 (0.001-2 U/ml) enhanced IEC-6
cell migration in a dose-dependent manner. Addition of 4
-bromophenacyl bromide (BPB) (a PLA2 inhibitor) to PLA2 completely
blocked the migration-promoting effect. However, addition of
piroxicam (a cyclooxygenase inhibitor) or nordihydroguaiaretic acid
(a lipoxygenase inhibitor) had no influence on the effect.
Lysophosphatidylcholine (lysoPC) (0.01-5000 ng/ml), one of the
products of phosphatidylcholine by PLA2, dose-dependently enhanced
IEC-6 cell migration as well. Combination of PLA2 (1 U/ml) and lysoPC
(1000 ng/ml) had no additive effect on migration. Moreover, the
migration-promoting effect of PLA2 which was blocked by BPB was
recovered by lysoPC. After pretreatment of IEC-6 cells with
replication-inhibiting doses of mitomycin C, the enhanced migration
induced by PLA2 or lysoPC was still observed. These observations
suggest that PLA2 may, independently of proliferation, enhance
intestinal epithelial cell migration mainly via lysoPC.
Received 11 September 1995; accepted in final form 9 April 1996.
APS Manuscript Number G402-5.
Article publication pending Am. J. Physiol. (Gastrointest. Liver
Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 8 May 96