Rhythmic oscillating complexes in the gastrointestinal tract of chickens: a role for motilin. Rodr[acute]iguez-Sinovas, A., M. Jim[acute]enez, P. De Clercq, T. L. Peeters, and P. Vergara. Department of Cell Biology and Physiology, Veterinary Faculty, Universitat Aut[grave]onoma de Barcelona, 08193 Bellaterra, Spain Gut Hormone Laboratory. Katholieke Universiteit Leuven, Belgium
APStracts 3:0218G, 1996.
Rhythmic oscillating complex (ROC) is a highly organized gastrointestinal motility pattern recently described in fasted avian species. ROCs show several high-speed propagated aborad contractions that progressively change into others of orad direction. In addition, chickens show migrating motor complexes (MMC) both in fed and fasting states. Recently, motilin was isolated and characterized from chicken small intestine. Accordingly, the aim of this study was to check if chicken motilin might be involved in either ROC or MMC induction. Electromyographical recordings were obtained from different areas of the gastrointestinal tract of chickens while motilin was infused. The response to chicken motilin was dose-dependent both in fed and fasted animals: a bolus of 4x10-11 moles/kg (n=5) did not modify the intestinal motor pattern, whereas 4x10-10 and 4x10-9 moles/kg (n=5 each) induced a complete ROC pattern of 5.2+/-0.6 and 10.8+/-0.9 minutes respectively. Rhythmic oscillating complexes induced by chicken motilin presented exactly the same pattern as that described during a spontaneous ROC. Furthermore, motilin concentration in plasma, measured by RIA, increased during a spontaneous ROC. This study suggests that chicken motilin triggers a rhythmic oscillating complex in chickens. The fact that plasma motilin levels increased during spontaneous ROC strongly suggests that motilin is involved in the induction of the ROC pattern. Motilin seems to play a different role in avian and mammalian species since a phase III of the MMC was never induced by motilin infusion.

Received 1 May 1996; accepted in final form 1 October 1996.
APS Manuscript Number G170-6.
Article publication pending Am. J. Physiol. (Gastrointest. Liver
Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 5 November 1996