Regulation of apolipoprotein secretion and lipid synthesis by fatty
acids in a newborn swine intestinal epithelial cell line.
Wang, Heng, Helen M. Berschneider, Jianhui Du, and Dennis D. Black.
Department of Pediatrics, Arkansas Children's Hospital Research
Institute, University of Arkansas for Medical Sciences, Little Rock,
AR 72202 and
APStracts 3:0224G, 1996.
The IPEC-1 newborn swine intestinal epithelial cell line was used to
determine the effects of the uptake of various fatty acids on the
secretion of apo B and A-I, as well as triglyceride and phospholipid.
Long-chain saturated fatty acids were taken up and stimulated
triglyceride synthesis, and palmitic (16:0) and stearic (18:0) acids
also stimulated phospholipid synthesis. However, these fatty acids
did not enhance triglyceride, phospholipid, or apo B or A-I
secretion. Oleic acid (18:1) was the most effective of all fatty
acids tested in stimulating triglyceride synthesis and the secretion
of triglyceride, phospholipid, and apo B. Linoleic (18:2) and
linolenic (18:3) acids were no more effective than long-chain
saturated fatty acids in stimulating these processes. With saturated
fatty acids, apo A-I followed the same secretory pattern as apo B.
However, among the unsaturated fatty acids, oleic acid was the least
effective and linolenic acid was the most effective in stimulating
apo A-I secretion. Basolateral secretion of lipid and apolipoproteins
by differentiated IPEC-1 cells is differentially regulated by apical
exposure to fatty acids.
Received 9 July 1996; accepted in final form 10 October 1996.
APS Manuscript Number G272-6.
Article publication pending Am. J. Physiol. (Gastrointest. Liver
Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 5 November 1996