Central inhibition of nitric oxide synthase modulates upper gastrointestinal motor activity. Ohta, Daisaku, Ching-Wen Lee, Sushil K. Sarna, Robert E. Condon, and Ivan M. Lang. Departments of Surgery, Physiology and Medicine, Medical College of Wisconsin and Zablocki VA Medical Center, Milwaukee, WI 53226
APStracts 3:0174G, 1996.
We investigated the role of central NO synthase inhibition in the modulation of fasting gastrointestinal motor activity and gastric emptying rate of solid nutrient meals in conscious dogs. N -L-nitro -arginine methyl ester (L-NAME) and N -nitro-L-arginine (L-NNA) were infused intracerebroventricularly. Circular muscle contractions were recorded with surgically implanted strain gauge transducers. Gastric emptying was measured by a radio-labeled meal. The inhibition of NO synthase in the brain suppressed phase III activity in the stomach and the duodenum but not in the jejunum or the ileum. However, the central inhibition of NO synthase had no significant effect on the rate of gastric emptying of solid nutrient meals or the duration of postprandial disruption of MMC cycling. The central actions of NO synthase inhibition were blocked by bilateral truncal vagotomy but not by intravenous administration of propranolol or phentolamine. We conclude that the inhibition of NO synthase inhibition in the brain generates a stimulus that selectively inhibits gastric and duodenal phase III activities. This stimulus, however, may not affect the postprandial gastroduodenal motor activity or the rate of gastric emptying of solid nutrient meals.

Received 21 February 1996; accepted in final form 27 August 1996.
APS Manuscript Number G65-6.
Article publication pending Am. J. Physiol. (Gastrointest. Liver
Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 19 September 1996