Neutrophils mediate the enhanced ischemia-reperfusion induced
capillary fluid filtration associated with hypercholesterolemia.
Harris, Norman R., and D. Neil Granger.
Department of Physiology and Biophysics, Louisiana State University
Medical Center, Shreveport, LA
APStracts 3:0146H, 1996.
Fluid filtration rate (Jv/S) from individual mesenteric capillaries in
normocholesterolemic and hypercholesterolemic rats was measured prior
to and following 30 minutes each of ischemia and reperfusion (I/R).
The median I/R-induced increase in Jv/S (I/R vs. baseline) was 44% in
normocholesterolemic rats (N=11) and 97% in hypercholesterolemic rats
(N=11). A positive correlation slope of .20% per mg/dl resulted when
plotting the % Jv/S increase versus plasma cholesterol concentration
(p=.02), demonstrating that hypercholesterolemia enhances the
capillary response to I/R. Since microvascular pressure did not
change significantly following I/R in either group of rats, the
increments in Jv/S likely reflect increased capillary permeability.
In hypercholesterolemic rats rendered neutropenic with antineutrophil
serum, I/R did not elicit a significant increase in Jv/S, suggesting
that activated neutrophils mediate the exaggerated endothelial
barrier dysfunction associated with hypercholesterolemia.
Received 7 November 1995; accepted in final form 29 March 1996.
APS Manuscript Number H1043-5.
Article publication pending Am. J. Physiol. (Heart Circ. Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 16 April 96