Skeletal muscle microvascular and tissue injury after varying
durations of ischemia.
Harris, A. G., R. Leiderer, F. Peer, and K. Messmer.
Institute for Surgical Research, University of Munich, Munich,
Germany
APStracts 3:0270H, 1996.
The purpose of this study was to examine the effect of varying
durations of ischemia on several microvascular parameters in the
awake hamster chamber model. The goal was to characterize the
microvascular damage which occurs in skeletal muscle as a result of
ischemia/reperfusion. The chamber tissues were subjected to one, two,
three, four, or five hours of ischemia and then the following
parameters were measured: vessel diameter, endothelial thickness,
macromolecular leakage, red blood cell (RBC) velocity, adherent
leukocytes, rolling leukocytes, freely flowing leukocytes, functional
capillary density and propidium iodide positive cell nuclei. In
control animals there was no significant difference in any parameters
over the entire observation period. After one or two hours of
ischemia a increase in rolling and adherent leukocytes was measured.
After three hours of ischemia there was a significant increase in the
mean endothelial thickness and in non-viable cells. After 4 hours of
ischemia a significant difference in the extent of macromolecular
leakage and the functional capillary density were additionally
observed. After 5 hours of ischemia this damage was more pronounced
and often so severe that approximately 50% of the vessels
demonstrated no-reflow.
Received 8 September 1995; accepted in final form 20 May 1996.
APS Manuscript Number H848-5.
Article publication pending Am. J. Physiol. (Heart Circ. Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 4 July 96