Fluid resuscitation with oxygen versus non-oxygen carriers after 2
-hour hemorrhagic shock in conscious hamsters.
Kerger, Heinz, Amy G. Tsai, Darin J. Saltzman, Robert M. Winslow, and
Marcos Intaglietta.
Department of Bioengineering and Department of Medicine, University
of California San Diego, La Jolla, CA 92093-0412
APStracts 3:0306H, 1996.
Efficacy of a cell-free o-raffinose cross-linked and oligomerized
hemoglobin (HemolinkTM) solution in restoring macro- and
microcirculatory conditions after 2-hour hemorrhagic shock (40 mmHg)
was compared to conventional treatment with autologous whole blood,
Ringer's Lactate (RL) and Dextran 70. Studies were conducted in the
dorsal skinfold microcirculation of conscious hamsters. Initial
infusion was equivalent to shed blood volume (SBV) for RL and 50% of
SBV for remaining solutions. After 2 hr all animals received blood,
50% of SBV. Vessel diameter, functional capillary density,
microvascular red blood cell velocity and flow were measured.
Arteriolar, venular and tissue pO2 were determined by phosphorescence
decay. Systemic parameters included mean arterial blood pressure
(MAP), heart rate, arterial blood gases, pH and base excess.
Autologous whole blood and HemolinkTM, but not Dextran 70 and RL
restored MAP, systemic blood gas and metabolic parameters. Tissue pO2
recovered to 40 - 50% with blood and HemolinkTM, but remained
significantly lower (10 - 15% of control) with Dextran 70 and RL.
Initial volume replacement after shock with blood or HemolinkTM
yields equivalent macro- and microhemodynamic improvements not
attainable with non-oxygen carrying plasma expanders.
Received 12 April 1996; accepted in final form 24 June 1996.
APS Manuscript Number H321-6.
Article publication pending Am. J. Physiol. (Heart Circ. Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 25 July 1996