Velocity differences between l-selectin and p-selectin dependent
neutrophil rolling in venules of the mouse cremaster muscle in
vivo.
Jung, Unsu, Daniel C. Bullard, Thomas F. Tedder, Klaus Ley.
Department of Biomedical Engineering, University of Virginia School
of Medicine, Charlottesville, VA 22908, Department of Molecular and
Human Genetics, Baylor College of Medicine and Howard Hughs Medical
Institute, Houston, TX 77030, Department of Immunology, Duke
University Medical Center, Durham, NC 27710
APStracts 3:0250H, 1996.
Following surgical trauma, leukocyte rolling is initially normal in L
-selectin deficient mice and reduced at later times, while leukocyte
rolling is initially absent in P-selectin deficient mice, but induced
later. Here, we examined the possibility that P- and L-selectin
support rolling at different characteristic velocities using
intravital microscopy of venules of the exteriorized cremaster muscle
venules of wild type (wt) and P- and L-selectin deficient mice. At
>50 min. after exteriorization, rolling in P-selectin deficient
mice occurred at significantly higher velocities (129+/-89 [mu]m/s)
than in wt mice (49+/-23 [mu]m/s). Rolling velocity distribution in
L-selectin deficient mice was similar to wt mice immediately after
exteriorization. Histological examination of Giemsa stained whole
-mount preparations in cremaster muscle venules revealed that the
majority of rolling cells (90% in all genotypes) were granulocytes.
We conclude that P-selectin mediates leukocyte rolling at velocities
below 50 [mu]m/s, while L-selectin sustains more rapid rolling. Under
physiological conditions, P- and L-selectin synergize to support
rolling at velocities between 20 and 70 [mu]m/s as seen in wt mice.
Received 14 February 1996; accepted in final form 7 June 1996.
APS Manuscript Number H153-6.
Article publication pending Am. J. Physiol. (Heart Circ. Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 28 June 96