Cytokine-induced leukocyte rolling in mouse cremaster muscle
arterioles is p-selectin-dependent.
Thorlacius, Henrik, Lennart Lindbom, and Johan Raud.
Department of Physiology & Pharmacology, Karolinska Institutet,
Stockholm, S-17177 Sweden
APStracts 3:0457H, 1996.
Leukocyte rolling and adhesion is generally observed in venules but
rarely in arterioles. By use of intravital microscopy, we found that
4 hour treatment with IL-1( and TNF-( dose-dependently induced
leukocyte rolling and adhesion in arterioles of the mouse cremaster
muscle. The rolling response lasted more than 24 hours and was
completely inhibited by treatment with the sulfated polysaccharide
fucoidin. Moreover, we found that costimulation with IL-1( and TNF-(
for 4 hours synergistically increased arteriolar leukocyte rolling,
i.e. threshold doses of IL-1( and TNF-( together caused a more than
10-fold increase of rolling in arterioles compared to the sum of the
individual responses. This rolling interaction was abolished by
treatment with a monoclonal antibody directed against P-selectin
(RB40.34) but apparently unaffected by a monoclonal antibody against
L-selectin (MEL-14). Taken together, our functional data show that
IL-1( and TNF-( separately induce and synergistically increase P
-selectin-dependent leukocyte rolling and firm adhesion in mouse
cremaster arterioles.
Received 8 August 1996; accepted in final form 17 October 1996.
APS Manuscript Number H720-6.
Article publication pending Am. J. Physiol. (Heart Circ. Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 5 November 1996