Neutrophil recruitment as a factor limiting injury or promoting
recovery from acute lung injury.
Carey, Lisa A., Sandra Z. Perkowski, Caren L. Lipsky, Rosemary A.
Cirelli, James A. Spath, Jr., and Marlys H. Gee.
Departments of Physiology, Medicine and Pediatrics, Division of
Pulmonary Medicine and Critical Care, Division of Neonatology,
Jefferson Medical College, Thomas Jefferson University, Philadelphia,
PA 19107 and Department of Clinical Studies, School of Veterinary
Medicine, University of Pennsylvania, Philadelphia, PA 19104
APStracts 3:0370H, 1996.
We studied (a) neutrophil mobilization in sheep given endotoxin (10
ng/kg/min, n = 5) for 4 hours, (b) surviving (n = 17) and
nonsurviving sheep (n = 8) during a 12 hour infusion of endotoxin,
and (c) adult sheep (n = 8) or lambs (n = 8) infused with endotoxin
for 12 h. Bone marrow cells of sheep declined from a baseline value
of 10,533 1784 to 5,966 1980 cells/ l (p < 0.05) 4 h after endotoxin.
After 12 h of endotoxin infusion, circulating neutrophils remained
reduced from baseline values of 2000-4000 cells/ l to 343 70 in lambs
and 484 236 nonsurviving sheep, while beginning to recover in
surviving sheep to 1838 467 cells/ l. In lambs and nonsurviving
sheep, a 12 h infusion of endotoxin increased lung lymph protein
clearance 10-fold compared to a 5-fold increase surviving in sheep.
Neutrophils cultured from sheep bone marrow exposed to lamb
-postendotoxin plasma failed to increase in cell number (609 229 to
610 182 cells/ l), while similar cells exposed to adult sheep post
-endotoxic plasma showed a significant increase in cell number (1069
101 to 2293 448 cells/ l, p < 0.05). Our results are consistent
with the hypothesis that the ability to recruit neutrophils to the
circulation during periods of inflammation is important in limiting
the severity of acute lung injury.
Received 6 November 1995; accepted in final form 29 July 1996.
APS Manuscript Number H1033-5.
Article publication pending Am. J. Physiol. (Heart Circ. Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 19 September 1996