Cardiomyocyte dna synthesis and binucleation during murine development. Soonpaa, Mark H., Kyung Keun Kim, Laura Pajak, Michael Franklin, and Loren J. Field. Krannert Institute of Cardiology, Indiana University School of Medicine, 1111 West 10th Street, Indianapolis, IN 46202-4800
APStracts 3:0375H, 1996.
Cardiomyocyte DNA synthesis and binucleation indices were determined during murine devel opment. Cardiomyocyte DNA synthesis occurred in two temporally distinct phases. The first phase occurred during fetal life and was associated exclusively with cardiomyocyte prolifera tion. The second phase occurred during early neonatal life and was associated with binuclea tion. Collectively, these results suggest that cardiomyocyte reduplication ceases during late fetal life. Northern and Western blot analyses identified several candidate genes which were differentially expressed during the reduplicative and binucleation phases of cardiomyocyte growth.

Received 16 April 1996; accepted in final form 22 August 1996.
APS Manuscript Number H336-6.
Article publication pending Am. J. Physiol. (Heart Circ. Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 19 September 1996