Airway closure and re-opening assessed by the alveolar capsule oscillation technique. Otis, David R., Ferenc Petak, Zoltan Hantos, Jeffrey Fredberg, and Roger D. Kamm. Fluid Mechanics Laboratory, Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, Departments of Medical Informatics and Experimental Surgery, Albert Szent-Gyorgyi Medical University, H-6720 Szeged, Hungary, Department of Environmental Health, Harvard School of Public Health, Boston, MA 02215
APStracts 3:0069A, 1996.
An alveolar capsule oscillation technique was used to determine (i) the lobe pressure and volume at which airways close and re-open, (ii) the effect of expiration rate on closing volume and pressure, (iii) the phase in the breathing cycle at which airway closure occurs and (iv) the site of airway closure. Experiments were conducted in excised dog lobes; closure was detected by an abrupt increase in the input impedance of surface-mounted alveolar capsules. Mean transpulmonary pressure at closure was slightly less than zero (Ptp 2.5 cm H2O); the corresponding mean re-opening pressure was Ptp 14 cm H2O. The expiration rate, varied between 1 and 20 %TLC/s, had no consistent effect on the closing volume and pressure. When lung volume was cycled up to frequencies of 0.2 Hz, closure generally occurred on expiration rather than inspiration. These observations lend support to the conclusion that mechanical collapse rather than meniscus formation is the most likely mechanism producing airway closure in normal, excised dog lungs. Analysis of measured acoustic impedances and re-opening pressures suggests that closure occurs in the most peripheral airways. Re-opening during inspiration was often observed to consist of a series of stepwise decreases in capsule impedance, indicating a sequence of opening events.

Received 24 February 1995; accepted in final form 16 January
1996.
APS Manuscript Number A220-5.
Article publication pending Journal of Applied Physiology.
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 8 February 96