APStracts 3:0044A, 1996.

Nitrovasodilator repletion increases tnf-[alpha] induced pulmonary edema. Johnson, Arnold, and Thomas J. Ferro. Research Service, Samuel S. Stratton Veterans Affairs Medical Center, Departments of Medicine, Physiology and Cell Biology, The Albany Medical College, Albany, NY 12208

APStracts 3:0044A, 1996.

We tested the hypothesis that nitrovasodilator repletion enhances tumor necrosis factor-[alpha] (TNF) induced pulmonary edema. Lungs were isolated from control guinea pigs or 4 hours after the intraperitoneal injection of TNF (1.60 x 105 U/kg). In the control and TNF lungs, the thromboxane mimetic U46619 (155 pmol/minute) caused increases in pulmonary capillary pressure (Ppc) and lung weight (_W). In control lungs, the nitrovasodilator agonist S -nitroso-N-acetyl-penicillamine (SNAP, 1 uM) attenuated the U46619 -induced increases in Ppc. In TNF lungs, SNAP had no effect on Ppc and increased _W. The ONOO- scavenger urate (35 mM) prevented the TNF+SNAP- induced increases in Ppc and _W. In addition, chemically synthesized peroxynitrite (OONO-, 4.0 mM) enhanced U46619-induced increases in _W. The data indicate that .NO repletion enhances TNF induced pulmonary edema possibly via OONO-.

Received 13 July 1995; accepted in final form 8 January 1996.
APS Manuscript Number A764-5.
Article publication pending Journal of Applied Physiology.
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 25 January 96