The effect of sprint cycle training on activities of antioxidant
enzymes in human skeletal muscle.
Hellsten, Ylva, Fred S. Apple, and Bertil Sj[diaeresis]odin.
Department of Physiology III, Karolinska Institute, Stockholm,
Sweden, Copenhagen Muscle Research Centre, August Krogh Institute,
Copenhagen, Denmark and Department of Laboratory Medicine and
Pathology, Hennepin County Medical Center, University of Minnesota
Medical School, Minneapolis, Minnesota, USA
APStracts 3:0266A, 1996.
The effect of intermittent sprint cycle training on the level of
muscle antioxidant enzyme protection was investigated. Resting muscle
biopsies, obtained prior to, after 6 weeks of training and 3, 24, and
72 h after the final session of an additional week of more frequent
training, were analyzed for activities of the antioxidant enzymes
glutathione peroxidase (GPX), glutathione reductase (GR), and
superoxide dismutase (SOD). Activities of several muscle metabolic
enzymes were determined to assess the effectiveness of the training.
After the first six week training period no change in GPX, GR or SOD
was observed but after the seventh week of training there was an
increase in GPX from 120+/-12 (+/-SE) to 164+/-24 [mu]mol . min-1 .
g-1 d.w. (P<0.05) and in GR from 10.8+/-0.8 to 16.8+/-2.4
[mu]mol . min-1 . g-1 d.w. (P<0.05). There was no significant
change in SOD. Sprint cycle training induced a significant
(P<0.05) elevation in the activity of phosphofructokinase and
creatine kinase implying an enhanced anaerobic capacity in the
trained muscle. The present study demonstrates that intermittent
sprint cycle training which induces an enhanced capacity for
anaerobic energy generation also improves the level of antioxidant
protection in the muscle.
Received 2 January 1996; accepted in final form 2 April 1996.
APS Manuscript Number A2-6.
Article publication pending Journal of Applied Physiology.
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 17 June 96