Maturational changes in responses of tissue and airway resistance to histamine. Dreshaj, Ismail A., Musa A. Haxhiu, Charles F. Potter, Faton H. Agani, Richard J. Martin. Department of Medicine, School of Medicine, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, Ohio 44106,
APStracts 3:0281A, 1996.
We determined how postnatal maturation affects the relative contributions of airways and lung parenchyma to pulmonary resistance, and whether there are developmental differences in their respective responses to constrictive agents. We studied open-chested, ventilated, anesthetized pigs at three ages: 2-4 days, 2-3 weeks, and 10 weeks. RL was partitioned into tissue (Rti) and airway (Raw) resistance by means of alveolar capsules under baseline conditions and following intravenous histamine. Postnatal maturation was associated with a progressive decline in RL, Rti and Raw, and increase in the contribution of Rti to RL from 38+/-8% at 2-4 days to 72+/-2% at both 2-3 and 10 weeks. Histamine caused RL to increase at all ages. When partitioned into Rti and Raw, the percent increase in Rti significantly exceeded that of Raw at both 2-4 days and 2-3 weeks. In contrast, the percent increase in Raw significantly exceeded that of Rti at 10 weeks. Administration of atropine prior to histamine in pigs at 10 weeks reduced the response of tissue and airway resistance to histamine. Histamine-induced responses of RL were blocked by prior H1 receptor blockade with pyrilamine (2 mg/kg). These results indicate that 1) the contribution of Rti and Raw to RL changes during maturation, and 2) contractile responses to exogenous histamine are manifest predominantly in most distal airways and lung parenchyma during early postnatal life; with advancing maturation there is greater contribution of airways to the increase in RL induced by histamine.

Received 31 July 1995; accepted in final form 31 May 1996.
APS Manuscript Number A838-5.
Article publication pending Journal of Applied Physiology.
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 28 June 96