In vivo iix and iib fiber recruitment in the gastrocnemius muscle of the rat is compartment related. Ruiter, C. J. De, P. E. M. H. Habets, A. De Haan, and A. J. Sargeant. Institute for Fundamental and Clinical Human Movement Sciences, Faculty of Human Movement Sciences, Vrije Universiteit, Amsterdam, The Netherlands
APStracts 3:0111A, 1996.
The purpose of the present study was to investigate to what extent fast twitch IIX and IIB fiber recruitment was related to the natural existing muscle compartments (subvolumes of muscle innervated by different primary nerve branches) in rat medial gastrocnemius (MG). Three groups (n=6) of rats trotted on a motor driven treadmill (20 degrees incline) at different speeds. A fourth group served as controls and a fifth group received in situ. electrical stimulation of all MG muscle fibers. Post exercise glycogen levels (periodic acid-Schiff (PAS) staining intensities) were made. Running caused more, and in situ stimulation caused less glycogen breakdown in the proximal IIX and IIB fibers compared to the fibers of the same type in the most distal compartment. Furthermore, the boundaries of the most distal compartment could often be recognized in the PAS stained cross-sections. It was concluded that during running the proximal IIX and IIB fibers were recruited to a greater extent (and at lower treadmill speeds) compared to the distal IIX and IIB fibers respectively.

Received 17 July 1995; accepted in final form 9 February 1996.
APS Manuscript Number A775-5.
Article publication pending Journal of Applied Physiology.
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 13 March 96