Capacity of circulating neutrophils to produce reactive oxygen
species following exhaustive exercise.
Suzuki[acute]e, Katsuhiko, Hideki Sato[acute]e, Takashi
Kikuchi[acute]e, Tatsuya Abe[acute]e, Shigeyuki Nakaji[acute]e, Kazuo
Sugawara[acute]e, Manabu Totsuka[acute]e, Koki Sato[acute]e, and
Kanemitsu Yamaya[acute]e.
Department of Hygiene, Hirosaki University School of Medicine, 5
Zaifu-cho, Hirosaki, Aomori 036, Japan; Department of Health and
Physical Education, Faculty of Education, Hirosaki University, 1
Bunkyo-cho, Hirosaki, Aomori 036, Japan; and ROyokyo Kidney Research
Institute, 90 Yamazaki, Kozawa, Hirosaki, Aomori 036, Japan
APStracts 3:0223A, 1996.
To investigate the cause of disagreement within the large number of
the literature concerning the effect of exercise on the capacity of
circulating neutrophils to produce reactive oxygen species (ROS), ten
male endurance-trained athletes underwent maximal exercise. The
generation of superoxide (O2-) by neutrophils was first detected on a
cell-by-cell basis using histochemical nitroblue tetrazolium (NBT)
tests performed directly on fresh unseparated blood, which showed
that responsive neutrophils under several stimulatory conditions
relatively decreased after exercise. Likewise, O2- detected with
lucigenin-dependent chemiluminescence (CL) of a fixed number of
purified neutrophils upon stimulation with opsonized zymosan was
decreased slightly following exercise. In contrast, the luminol
-dependent CL response of the neutrophils indicative of the
myeloperoxidase (MPO)-mediated formation of highly reactive oxidants
was significantly enhanced following exercise. It therefore suggests
that the pathway of neutrophil ROS metabolism might be forwarded from
the precursor O2- production to the stages of more reactive oxidants
formation. In addition, these phenomena were closely associated with
the exercise-induced mobilization of neutrophils from the marginated
pool into the circulation, which was mediated by the overshooting of
catecholamines during exercise. These findings indicate that the use
of different techniques for detecting ROS, or the different stages of
neutrophil ROS metabolism could explain some of the disparate
findings of the previous studies.
Received 26 January 1996; accepted in final form 22 April 1996.
APS Manuscript Number A86-6.
Article publication pending Journal of Applied Physiology.
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 8 May 96