Effect of lung inflation in vivo on airways with smooth muscle tone or edema Brown, ]obert H., Wayne Mitzner, Yonca Bulut, Elizabeth M. Wagner. Department of Anesthesiology and Critical Care Medicine, Department of Radiology, Department of Environmental Health Sciences/Division of Physiology, Department of Medicine, Division of Pulmonary and Critical Care Medicine
APStracts 3:0482A, 1996.
Fibrous attachments to the airway wall and a subpleural surrounding pressure can create an external load against which airway smooth muscle must contract. A decrease in this load has been proposed as a possible cause of increased airway narrowing in asthmatics. To study the interaction between the airways and the surrounding lung parenchyma, we investigated the effect of lung inflation on relaxed airways, airways contracted with methacholine, and airways made edematous by infusing bradykinin into the bronchial artery. Measurements were made in anesthetized sheep using high resolution computed tomography (HRCT) to visualize changes in individual airways. During methacholine infusion airway area was decreased, but increased minimally with increases in transpulmonary pressure. Bradykinin infusion caused a 50% increase in airway wall area and a small decrease in airway lumenal area. In contrast to airways contracted with methacholine, the lumenal area after bradykinin increased substantially with increases in transpulmonary pressure, reaching 99% of the relaxed area at TLC. Thus, airway edema by itself did not prevent full distension of the airway at lung volumes approaching TLC. Therefore, we speculate that, if a deep inspiration fails to relieve airway narrowing in vivo, this must be a manifestation of airway smooth muscle contraction and not airway wall edema.

Received 26 March 1996; accepted in final form 9 October 1996.
APS Manuscript Number A298-6.
Article publication pending Journal of Applied Physiology.
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 13 November 1996