Enhancement of intestinal water absorption and sodium transport by glycerol, in the rat. Wapnir, Raul A., Maria C. Sia, and Stanley E. Fisher. Divisions of Perinatal Medicine and Gastroenterology, Department of Pediatrics, North Shore University Hospital - Cornell University Medical College, Manhasset, New York 11030
APStracts 3:0402A, 1996.
Glycerol is a hydrophilic, absorbable and energy-rich solute which could make water absorption more efficient. We investigated the use of glycerol (GLY) in a high-energy beverage containing corn syrup (CS) using a small intestinal perfusion procedure in the rat, an approach shown earlier to provide good pre-clinical information. The effectiveness of several formulations with glycerol and CS was compared to commercial products and to experimental formulas where glycerol substituted for glucose (GLU). The CS-GLY combination was more effective than preparations on the market containing sucrose and glucose-fructose syrups (G-P and G-L) in maintaining a net water absorption balance in the test jejunal segment (means +/- SE: CS-GLY= 0.021 +/- 0.226, G-L= -1.516 +/- 0.467, and G-P= -0.299 +/- 0.106 [mu]L/min x cm [P=0.0113]) and in reducing sodium release into the lumen (CS-GLY = -133.2 +/- 16.2, G-L = -226.7 +/- 25.2, and G-P= -245.6 +/- 23.4 nmol/min x cm [P=0.0022]). In other preparations, at equal CS concentrations (60 and 80 g/L), GLY clearly improved net water absorption over a comparable GLU-containing product (CS60-GLY= 0.422 +/- 0.136 and CS80-GLY= 0.666 +/- 0.378 vs. CS60-GLU= -0.282 +/- 0.200 and CS80-GLU=-1.046 +/- 0.480 [mu]L/min x cm [P=0.0019]. Based on the data of this rat intestine perfusion model, GLY could be a useful ingredient in energy-rich beverages and might enhance fluid absorption in humans.

Received 22 November 1995; accepted in final form 16 August 1996.
APS Manuscript Number A1219-5.
Article publication pending Journal of Applied Physiology.
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 19 September 1996